PMID:15100018
| Citation |
Byrdin, M, Sartor, V, Eker, AP, Vos, MH, Aubert, C, Brettel, K and Mathis, P (2004) Intraprotein electron transfer and proton dynamics during photoactivation of DNA photolyase from E. coli: review and new insights from an "inverse" deuterium isotope effect. Biochim. Biophys. Acta 1655:64-70 |
|---|---|
| Abstract |
We review our work on electron transfer and proton dynamics during photoactivation in DNA photolyase from E. coli and discuss a recent theoretical study on this issue. In addition, we present unpublished data on the charge recombination between the fully reduced FADH(-) and the neutral (deprotonated) radical of the solvent exposed tryptophan W306. We found a pronounced acceleration with decreasing pH and an inverse deuterium isotope effect (k(H)/k(D)=0.35 at pL 6.5) and interpret it in a model of a fast protonation equilibrium for the W306 radical. Due to this fast equilibrium, two parallel recombination channels contribute differently at different pH values: one where reprotonation of the W306 radical is followed by electron transfer from FADH(-) (electron transfer time constant tau(et) in the order of 10-50 micros), and one where electron transfer from FADH(-) (tau(et)=25 ms) is followed by reprotonation of the W306 anion. |
| Links |
PubMed Online version:10.1016/j.bbabio.2003.07.001 |
| Keywords |
Deoxyribodipyrimidine Photo-Lyase/chemistry; Deoxyribodipyrimidine Photo-Lyase/metabolism; Deoxyribodipyrimidine Photo-Lyase/radiation effects; Deuterium/chemistry; Electron Transport; Energy Metabolism; Escherichia coli/enzymology; Kinetics; Models, Biological; Photobiology; Proton-Motive Force; Spectrophotometry; Tryptophan/chemistry |
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