PMID:15096523
| Citation |
van der Laan, M, Bechtluft, P, Kol, S, Nouwen, N and Driessen, AJ (2004) F1F0 ATP synthase subunit c is a substrate of the novel YidC pathway for membrane protein biogenesis. J. Cell Biol. 165:213-22 |
|---|---|
| Abstract |
The Escherichia coli YidC protein belongs to the Oxa1 family of membrane proteins that have been suggested to facilitate the insertion and assembly of membrane proteins either in cooperation with the Sec translocase or as a separate entity. Recently, we have shown that depletion of YidC causes a specific defect in the functional assembly of F1F0 ATP synthase and cytochrome o oxidase. We now demonstrate that the insertion of in vitro-synthesized F1F0 ATP synthase subunit c (F0c) into inner membrane vesicles requires YidC. Insertion is independent of the proton motive force, and proteoliposomes containing only YidC catalyze the membrane insertion of F0c in its native transmembrane topology whereupon it assembles into large oligomers. Co-reconstituted SecYEG has no significant effect on the insertion efficiency. Remarkably, signal recognition particle and its membrane-bound receptor FtsY are not required for the membrane insertion of F0c. In conclusion, a novel membrane protein insertion pathway in E. coli is described in which YidC plays an exclusive role. |
| Links |
PubMed PMC2172039 Online version:10.1083/jcb.200402100 |
| Keywords |
Adenosine Triphosphatases/metabolism; Bacterial Proteins/metabolism; Cell Membrane/chemistry; Cell Membrane/metabolism; Escherichia coli Proteins/metabolism; Liposomes/chemistry; Liposomes/metabolism; Macromolecular Substances; Membrane Potentials; Membrane Proteins/biosynthesis; Membrane Transport Proteins/metabolism; Mitochondrial Proton-Translocating ATPases/chemistry; Mitochondrial Proton-Translocating ATPases/metabolism; Models, Molecular; Protein Structure, Secondary; Protein Subunits/metabolism; Protons; Signal Recognition Particle/metabolism; Transport Vesicles/chemistry; Transport Vesicles/metabolism |
| edit table |
Significance
You can help EcoliWiki by summarizing why this paper is useful
Useful Materials and Methods
You can help Ecoliwiki by describing the useful materials (strains, plasmids, antibodies, etc) described in this paper.
Annotations
<annotationlinks/>
EcoliWiki Links
Add links to pages that link here (e.g. gene, product, method pages)
References
See Help:References for how to manage references in EcoliWiki.