PMID:15080672
| Citation |
Sikorski, RS, Wang, L, Markham, KA, Rajagopalan, PT, Benkovic, SJ and Kohen, A (2004) Tunneling and coupled motion in the Escherichia coli dihydrofolate reductase catalysis. J. Am. Chem. Soc. 126:4778-9 |
|---|---|
| Abstract |
H-transfer was studied in the complex kinetic cascade of dihydrofolate reductase. Intrinsic kinetic isotope effects, their temperature dependence, and other temperature-dependent parameters indicated H-tunneling, but no 1 degrees to 2 degrees coupled motion. The data also suggested environmentally coupled tunneling and commitment to catalysis on pre-steady-state isotope effects. |
| Links |
PubMed Online version:10.1021/ja031683w |
| Keywords |
Catalysis; Escherichia coli/enzymology; Escherichia coli/metabolism; Kinetics; Tetrahydrofolate Dehydrogenase/metabolism |
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