PMID:15071046
| Citation |
Johnson, JR and O'Bryan, TT (2004) Detection of the Escherichia coli group 2 polysaccharide capsule synthesis Gene kpsM by a rapid and specific PCR-based assay. J. Clin. Microbiol. 42:1773-6 |
|---|---|
| Abstract |
A rapid and simple PCR-based assay for detection of the group 2 capsule synthesis gene kpsM of Escherichia coli was designed and validated. When combined with the published group 2 primers (kpsIIf, 5'-GCGCATTTGCTGATACTGTTG-3'; kpsIIr, 5'-CATCCAGACGATAAGCATGAGCA-3'), the new primers (the kpsIIf primer and a new reverse primer K2r, 5'-AGGTAGTTCAGACTCACACCT-3') allowed specific identification by exclusion of the heretofore elusive K2 kpsM variant. The primers yielded the predicted amplicon when multiplexed with other primers and used under varied assay conditions, including a range of concentrations of individual reaction mixture ingredients and of annealing temperatures (from 54 to 64 degrees C). |
| Links | |
| Keywords |
Bacterial Capsules/metabolism; DNA Primers; Escherichia coli/classification; Escherichia coli/genetics; Escherichia coli Proteins/genetics; Genotype; Humans; Membrane Transport Proteins/genetics; Phenotype; Polymerase Chain Reaction/methods; Species Specificity; Time Factors |
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