PMID:15044727

Citation	Rapp, M, Drew, D, Daley, DO, Nilsson, J, Carvalho, T, Melén, K, De Gier, JW and Von Heijne, G (2004) Experimentally based topology models for E. coli inner membrane proteins. Protein Sci. 13:937-45
Abstract	Membrane protein topology predictions can be markedly improved by the inclusion of even very limited experimental information. We have recently introduced an approach for the production of reliable topology models based on a combination of experimental determination of the location (cytoplasmic or periplasmic) of a protein's C terminus and topology prediction. Here, we show that determination of the location of a protein's C terminus, rather than some internal loop, is the best strategy for large-scale topology mapping studies. We further report experimentally based topology models for 31 Escherichia coli inner membrane proteins, using methodology suitable for genome-scale studies.
Links	PubMed PMC2280059 Online version:10.1110/ps.03553804
Keywords	Alkaline Phosphatase; Cell Membrane/chemistry; Cell Membrane/genetics; Computational Biology; Cyclin-Dependent Kinases/chemistry; Cyclin-Dependent Kinases/genetics; Escherichia coli/chemistry; Escherichia coli/genetics; Escherichia coli Proteins/chemistry; Escherichia coli Proteins/genetics; Gene Expression; Green Fluorescent Proteins; Luminescent Proteins; Recombinant Fusion Proteins/chemistry; Recombinant Fusion Proteins/genetics; Software
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