PMID:15044024

Citation	Ninio, S, Elbaz, Y and Schuldiner, S (2004) The membrane topology of EmrE - a small multidrug transporter from Escherichia coli. FEBS Lett. 562:193-6
Abstract	EmrE is a multidrug transporter from Escherichia coli that belongs to the Smr family of small multidrug transporters. The secondary structure of EmrE consists of a four helical bundle, as judged by different techniques. EmrE has been extensively characterized; nevertheless, the membrane topology of EmrE has not been determined yet. Previous work with a homologous Smr protein provided partial information of the membrane topology, however the location of the carboxy-terminus remained inconclusive. In this work we probed the membrane topology of EmrE, focusing on the carboxy-terminus of the protein, using two independent approaches. Our results support a secondary structure where the carboxy-terminus faces the cytoplasm, while the first loop faces the periplasm.
Links	PubMed Online version:10.1016/S0014-5793(04)00240-6
Keywords	Amino Acid Sequence; Antiporters/chemistry; Antiporters/metabolism; Cell Membrane/chemistry; Escherichia coli Proteins/chemistry; Escherichia coli Proteins/metabolism; Membrane Proteins/chemistry; Membrane Proteins/metabolism; Molecular Sequence Data; Protein Structure, Secondary; Sulfhydryl Compounds/chemistry
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