PMID:15039583
| Citation |
Ericsson, UB, Andersson, ME, Engvall, B, Nordlund, P and Hallberg, BM (2004) Expression, purification, crystallization and preliminary diffraction studies of the tRNA pseudouridine synthase TruD from Escherichia coli. Acta Crystallogr. D Biol. Crystallogr. 60:775-6 |
|---|---|
| Abstract |
Pseudouridine, the 5-ribosyl isomer of uridine, is the most common modification of structural RNA. The recently identified pseudouridine synthase TruD belongs to a widespread class of pseudouridine synthases without significant sequence homology to previously known families. TruD from Escherichia coli was overexpressed, purified and crystallized. The crystals diffract to a minimum Bragg spacing of 2.4 A and belong to space group P2(1)2(1)2(1), with unit-cell parameters a = 63.4, b = 108.6, c = 111.7 A. |
| Links |
PubMed Online version:10.1107/S0907444904003506 |
| Keywords |
Cloning, Molecular; Crystallization; Crystallography, X-Ray; Escherichia coli Proteins/chemistry; Hydro-Lyases/chemistry; Intramolecular Transferases/chemistry |
| edit table |
Significance
You can help EcoliWiki by summarizing why this paper is useful
Useful Materials and Methods
You can help Ecoliwiki by describing the useful materials (strains, plasmids, antibodies, etc) described in this paper.
Annotations
<annotationlinks/>
EcoliWiki Links
Add links to pages that link here (e.g. gene, product, method pages)
References
See Help:References for how to manage references in EcoliWiki.