PMID:14998992
| Citation |
Tzagoloff, A, Barrientos, A, Neupert, W and Herrmann, JM (2004) Atp10p assists assembly of Atp6p into the F0 unit of the yeast mitochondrial ATPase. J. Biol. Chem. 279:19775-80 |
|---|---|
| Abstract |
The F(0)F(1)-ATPase complex of yeast mitochondria contains three mitochondrial and at least 17 nuclear gene products. The coordinate assembly of mitochondrial and cytosolic translation products relies on chaperones and specific factors that stabilize the pools of some unassembled subunits. Atp10p was identified as a mitochondrial inner membrane component necessary for the biogenesis of the hydrophobic F(0) sector of the ATPase. Here we show that, following its synthesis on mitochondrial ribosomes, subunit 6 of the ATPase (Atp6p) can be cross-linked to Atp10p. This interaction is required for the integration of Atp6p into a partially assembled subcomplex of the ATPase. Pulse labeling and chase of mitochondrial translation products in vivo indicate that Atp6p is less stable and more rapidly degraded in an atp10 null mutant than in wild type. Based on these observations, we propose Atp10p to be an Atp6p-specific chaperone that facilitates the incorporation of Atp6p into an intermediate subcomplex of ATPase subunits. |
| Links |
PubMed Online version:10.1074/jbc.M401506200 |
| Keywords |
Adenosine Triphosphatases/chemistry; Cross-Linking Reagents/pharmacology; Cytosol/metabolism; Electrophoresis, Polyacrylamide Gel; Mitochondria/metabolism; Mitochondria/pathology; Mitochondrial Proton-Translocating ATPases; Models, Biological; Molecular Chaperones/chemistry; Molecular Chaperones/physiology; Mutation; Precipitin Tests; Protein Binding; Protein Biosynthesis; Protein Structure, Tertiary; Proton-Translocating ATPases/chemistry; Saccharomyces cerevisiae/metabolism; Saccharomyces cerevisiae Proteins/chemistry; Saccharomyces cerevisiae Proteins/physiology; Time Factors |
| edit table |
Significance
You can help EcoliWiki by summarizing why this paper is useful
Useful Materials and Methods
You can help Ecoliwiki by describing the useful materials (strains, plasmids, antibodies, etc) described in this paper.
Annotations
<annotationlinks/>
EcoliWiki Links
Add links to pages that link here (e.g. gene, product, method pages)
References
See Help:References for how to manage references in EcoliWiki.