PMID:14981317
| Citation |
Ohtsu, I, Kakuda, N, Tsukagoshi, N, Dokyu, N, Takagi, H, Wachi, M and Aono, R (2004) Transcriptional analysis of the ostA/imp gene involved in organic solvent sensitivity in Escherichia coli. Biosci. Biotechnol. Biochem. 68:458-61 |
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| Abstract |
Transcriptional initiation sites of the ostA gene involved in organic solvent sensitivity in Escherichia coli were found by primer extension analysis. Two transcriptional initiation sites were newly identified at -133 and -48 nucleotides from the initiation codon of ostA, but the previously reported sigmaE-dependent one at -227 could not be detected. No heat-inducible expression of ostA was observed by Northern blotting analysis, indicating that the contribution of sigmaE-dependent transcription was very small if any. SigmaD-dependent promoter-like sequences were found just upstream of the newly identified transcriptional initiation sites by computer-aided analysis. Deletion analysis of ostA-lacZ fusions demonstrated that these two promoters contributed almost equally to the constitutive expression of the ostA gene. |
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| Keywords |
Bacterial Outer Membrane Proteins/genetics; Blotting, Northern; Chromosome Mapping; DNA Primers; Escherichia coli/drug effects; Escherichia coli/genetics; Escherichia coli Proteins/genetics; Genes, Bacterial/genetics; Lac Operon/genetics; Promoter Regions, Genetic/genetics; RNA, Bacterial/analysis; RNA, Bacterial/genetics; Recombinant Fusion Proteins/genetics; Solvents/pharmacology; Transcriptional Activation/genetics |
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