PMID:1484484
| Citation |
Ishihama, A (1992) Role of the RNA polymerase alpha subunit in transcription activation. Mol. Microbiol. 6:3283-8 |
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| Abstract |
The N-terminal two-thirds of the alpha subunit of Escherichia coli RNA polymerase plays an essential role in the initiation of subunit assembly, by gathering two large subunits, beta and beta', together into a core-enzyme complex. One group of RNA polymerase mutants deficient in response to transcription activation carries mutations in the C-terminal region of the alpha subunit, indicating that the C-terminal region of the alpha subunit is involved in protein-protein contact in positive control of transcription. A set of activators (class I transcription factors) which make contact with this contact site I region on RNA polymerase alpha subunit bind in most cases to DNA upstream of the promoter -35 signal. Genetic fine mapping indicates that a cluster of subsites exists in the contact site I region, each interacting with a set of the class I factors and each consisting of a structure formed by only 5-10 amino acid residues. |
| Links | |
| Keywords |
Bacterial Proteins/metabolism; Binding Sites; DNA, Bacterial/metabolism; Escherichia coli/genetics; Promoter Regions, Genetic; Protein Conformation; RNA Polymerase I/chemistry; RNA Polymerase I/physiology; Regulatory Sequences, Nucleic Acid; Transcription Factors/metabolism; Transcription, Genetic |
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