PMID:14766310
| Citation |
Xu, Y, Wen, D, Clancy, P, Carr, PD, Ollis, DL and Vasudevan, SG (2004) Expression, purification, crystallization, and preliminary X-ray analysis of the N-terminal domain of Escherichia coli adenylyl transferase. Protein Expr. Purif. 34:142-6 |
|---|---|
| Abstract |
A soluble N-terminal domain of the Escherichia coli adenylyl transferase (ATase) is responsible for deadenylylation activity of the intact enzyme. Previous studies of the deadenylylation activity have involved a fragment, AT-N423 (residues 1 to 423), which was extended by 17 amino acids to give AT-N440. This new domain is truncated at the end of a predicted helix and prior to a Q-linker. The domain was found to be very soluble and stable so that it could be purified to homogeneity and crystallized. This construct has deadenylylation activity that is independent of the low nitrogen status indicator PII-UMP. The crystals belong to space group P3(1)21 or its enantiomorph P3(2)21 with a=b=116.6 A and c=67.6 A. |
| Links |
PubMed Online version:10.1016/j.pep.2003.11.001 |
| Keywords |
Adenosine Monophosphate/metabolism; Amino Acid Sequence; Anion Exchange Resins/chemistry; Blotting, Western; Chromatography/methods; Crystallization; Crystallography, X-Ray; Electrophoresis, Polyacrylamide Gel; Escherichia coli/enzymology; Escherichia coli/genetics; Gene Expression/genetics; Genetic Vectors/genetics; Glutamate-Ammonia Ligase/metabolism; Glutamates/analysis; Glutamates/metabolism; Hydroxamic Acids/analysis; Hydroxamic Acids/metabolism; Ketoglutaric Acids/chemistry; Ketoglutaric Acids/metabolism; Molecular Sequence Data; Nucleotidyltransferases/biosynthesis; Nucleotidyltransferases/chemistry; Nucleotidyltransferases/isolation & purification; Nucleotidyltransferases/metabolism; PII Nitrogen Regulatory Proteins; Peptide Fragments/biosynthesis; Peptide Fragments/chemistry; Protein Structure, Secondary; Recombinant Proteins/biosynthesis; Recombinant Proteins/isolation & purification; Recombinant Proteins/metabolism; Sepharose/analogs & derivatives; Sepharose/chemistry |
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