PMID:14763860
| Citation |
D'Auria, S, Alfieri, F, Staiano, M, Pelella, F, Rossi, M, Scirè, A, Tanfani, F, Bertoli, E, Grycznyski, Z and Lakowicz, JR Structural and thermal stability characterization of Escherichia coli D-galactose/D-glucose-binding protein. Biotechnol. Prog. 20:330-7 |
|---|---|
| Abstract |
The effect of temperature and glucose binding on the structure of the galactose/glucose-binding protein from Escherichia coli was investigated by circular dichroism, Fourier transform infrared spectroscopy, and steady-state and time-resolved fluorescence. The data showed that the glucose binding induces a moderate change of the secondary structure content of the protein and increases the protein thermal stability. The infrared spectroscopy data showed that some protein stretches, involved in alpha-helices and beta strand conformations, are particularly sensitive to temperature. The fluorescence studies showed that the intrinsic tryptophanyl fluorescence of the protein is well represented by a three-exponential model and that in the presence of glucose the protein adopts a structure less accessible to the solvent. The new insights on the structural properties of the galactose/glucose-binding protein can contribute to a better understanding of the protein functions and represent fundamental information for the development of biotechnological applications of the protein. |
| Links |
PubMed Online version:10.1021/bp0341848 |
| Keywords |
Computer Simulation; Escherichia coli/metabolism; Escherichia coli Proteins/analysis; Escherichia coli Proteins/chemistry; Models, Molecular; Monosaccharide Transport Proteins/analysis; Monosaccharide Transport Proteins/chemistry; Protein Conformation; Protein Structure, Secondary; Temperature |
| edit table |
Significance
You can help EcoliWiki by summarizing why this paper is useful
Useful Materials and Methods
You can help Ecoliwiki by describing the useful materials (strains, plasmids, antibodies, etc) described in this paper.
Annotations
<annotationlinks/>
EcoliWiki Links
Add links to pages that link here (e.g. gene, product, method pages)
References
See Help:References for how to manage references in EcoliWiki.