PMID:14755567

Citation	Skibinska, L, Banachowicz, E, Gapiński, J, Patkowski, A and Barciszewski, J (2004) Structural similarity of E. coli 5S rRNA in solution and within the ribosome. Biopolymers 73:316-25
Abstract	The article presents translational and rotational diffusion coefficients of 5S rRNA determined experimentally by the method of dynamic light scattering (DLS) and its comparison with the values predicted for different models of this molecule. The tertiary structure of free 5S rRNA was proposed on the basis of the atomic structures of the 5S rRNA from E. coli and H. marismortui extracted from the ribosome. A comparison of the values of DT, tauR, and Rg predicted for different models with experimental results for the free molecule in solution suggests that free 5S rRNA is less compact than that in the complex with ribosomal proteins. In general, the molecules of 5S rRNA consist of three domains: a short one and two longer ones. As follows from a comparison of the results of our simulations with experimental values, in the molecule in solution the two closest helical fragments of the longer domains remain collinear, whereas the short domain takes a position significantly deviated from them.
Links	PubMed Online version:10.1002/bip.10598
Keywords	Base Sequence; Escherichia coli/chemistry; Escherichia coli/genetics; Light; Models, Molecular; Molecular Sequence Data; Nucleic Acid Conformation; RNA, Bacterial/chemistry; RNA, Bacterial/genetics; RNA, Ribosomal, 5S/chemistry; RNA, Ribosomal, 5S/genetics; Ribosomes/chemistry; Scattering, Radiation; Solutions
edit table

Significance

You can help EcoliWiki by summarizing why this paper is useful

Useful Materials and Methods

You can help Ecoliwiki by describing the useful materials (strains, plasmids, antibodies, etc) described in this paper.

Annotations

<annotationlinks/>

EcoliWiki Links

Add links to pages that link here (e.g. gene, product, method pages)

References

See Help:References for how to manage references in EcoliWiki.