PMID:14742217
| Citation |
Pfeiffer, ES and Hiasa, H (2004) Replacement of ParC alpha4 helix with that of GyrA increases the stability and cytotoxicity of topoisomerase IV-quinolone-DNA ternary complexes. Antimicrob. Agents Chemother. 48:608-11 |
|---|---|
| Abstract |
Replacement of the alpha4 helix of ParC with that of GyrA increased the stability of topoisomerase IV-quinolone-DNA ternary complexes. This mutant topoisomerase IV-mediated cell killing was more efficient than topoisomerase IV-mediated cell killing in Escherichia coli. Thus, the alpha4 helix plays critical roles in determining the stability and the cytotoxicity of ternary complexes. |
| Links | |
| Keywords |
Anti-Infective Agents/pharmacology; Cloning, Molecular; DNA Gyrase/chemistry; DNA Gyrase/genetics; DNA Topoisomerase IV/chemistry; DNA Topoisomerase IV/genetics; DNA, Bacterial/chemistry; DNA, Bacterial/genetics; Drug Resistance, Bacterial; Escherichia coli/drug effects; Escherichia coli/genetics; Mutation/genetics; Norfloxacin/pharmacology; Quinolones/chemistry |
| edit table |
Significance
You can help EcoliWiki by summarizing why this paper is useful
Useful Materials and Methods
You can help Ecoliwiki by describing the useful materials (strains, plasmids, antibodies, etc) described in this paper.
Annotations
<annotationlinks/>
EcoliWiki Links
Add links to pages that link here (e.g. gene, product, method pages)
References
See Help:References for how to manage references in EcoliWiki.