PMID:14731399
| Citation |
Dinos, G, Wilson, DN, Teraoka, Y, Szaflarski, W, Fucini, P, Kalpaxis, D and Nierhaus, KH (2004) Dissecting the ribosomal inhibition mechanisms of edeine and pactamycin: the universally conserved residues G693 and C795 regulate P-site RNA binding. Mol. Cell 13:113-24 |
|---|---|
| Abstract |
The crystal structures of the universal translation-initiation inhibitors edeine and pactamycin bound to ribosomal 30S subunit have revealed that edeine induces base pairing of G693:C795, residues that constitute the pactamycin binding site. Here, we show that base pair formation by addition of edeine inhibits tRNA binding to the P site by preventing codon-anticodon interaction and that addition of pactamycin, which rebreaks the base pair, can relieve this inhibition. In addition, edeine induces translational misreading in the A site, at levels comparable to those induced by the classic misreading antibiotic streptomycin. Binding of pactamycin between residues G693 and C795 strongly inhibits translocation with a surprising tRNA specificity but has no effect on translation initiation, suggesting that reclassification of this antibiotic is necessary. Collectively, these results suggest that the universally conserved G693:C795 residues regulate tRNA binding at the P site of the ribosome and influence translocation efficiency. |
| Links | |
| Keywords |
Anticodon/metabolism; Base Pairing; Binding Sites; Codon/metabolism; Cytosine/metabolism; Edeine/pharmacology; Escherichia coli; Green Fluorescent Proteins; Guanine/metabolism; Luminescent Proteins/metabolism; Models, Biological; Models, Molecular; Nucleic Acid Synthesis Inhibitors/pharmacology; Pactamycin/pharmacology; Protein Biosynthesis; Protein Synthesis Inhibitors/pharmacology; RNA/metabolism; RNA, Ribosomal/metabolism; RNA, Transfer/metabolism; Ribosomal Proteins/metabolism; Ribosomes/drug effects; Ribosomes/metabolism |
| edit table |
Significance
You can help EcoliWiki by summarizing why this paper is useful
Useful Materials and Methods
You can help Ecoliwiki by describing the useful materials (strains, plasmids, antibodies, etc) described in this paper.
Annotations
<annotationlinks/>
EcoliWiki Links
Add links to pages that link here (e.g. gene, product, method pages)
References
See Help:References for how to manage references in EcoliWiki.