PMID:14702328
| Citation |
Self, WT, Hasona, A and Shanmugam, KT (2004) Expression and regulation of a silent operon, hyf, coding for hydrogenase 4 isoenzyme in Escherichia coli. J. Bacteriol. 186:580-7 |
|---|---|
| Abstract |
On the basis of hyf-lacZ fusion studies, the hyf operon of Escherichia coli, noted for encoding the fourth hydrogenase isoenzyme (HYD4), is not expressed at a significant level in a wild-type strain. However, mutant FhlA proteins (constitutive activators of the hyc-encoded hydrogenase 3 isoenzyme) activated hyf-lacZ. HyfR, an FhlA homolog encoded by the hyfR gene present at the end of the hyf operon, also activated transcription of hyf-lacZ but did so only when hyfR was expressed from a heterologous promoter. The HYD4 isoenzyme did not substitute for HYD3 in H(2) production. Optimum expression of hyf-lacZ required the presence of cyclic AMP receptor protein-cyclic AMP complex and anaerobic conditions when HyfR was the activator. |
| Links | |
| Keywords |
Aerobiosis; Base Sequence; Cyclic AMP/physiology; Escherichia coli/enzymology; Escherichia coli/genetics; Escherichia coli/growth & development; Escherichia coli Proteins; Formates/pharmacology; Gene Expression Regulation, Bacterial; Hydrogenase/genetics; Molecular Sequence Data; Molybdenum/pharmacology; Operon; Transcription, Genetic |
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