PMID:14687566
| Citation |
Westblade, LF, Ilag, LL, Powell, AK, Kolb, A, Robinson, CV and Busby, SJ (2004) Studies of the Escherichia coli Rsd-sigma70 complex. J. Mol. Biol. 335:685-92 |
|---|---|
| Abstract |
Escherichia coli Rsd protein was previously identified on the basis of its binding to the RNA polymerase sigma(70) subunit. The Rsd-sigma(70) complex has been studied using different methods. Our data show that Rsd associates with sigma(70) to form a complex with a stoichiometry of 1:1. Alanine scanning and deletion mutagenesis were used to locate regions of sigma(70) that are required for the formation of the Rsd-sigma(70) complex. |
| Links | |
| Keywords |
Binding Sites; DNA-Directed RNA Polymerases/chemistry; DNA-Directed RNA Polymerases/genetics; DNA-Directed RNA Polymerases/metabolism; Electrophoresis, Polyacrylamide Gel; Escherichia coli Proteins/chemistry; Escherichia coli Proteins/metabolism; Molecular Weight; Mutagenesis, Site-Directed; Protein Binding; Repressor Proteins/chemistry; Repressor Proteins/metabolism; Sigma Factor/chemistry; Sigma Factor/genetics; Sigma Factor/metabolism; Spectrometry, Mass, Electrospray Ionization |
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