PMID:14685649

Citation	Hambraeus, G and Rutberg, B (2004) Escherichia coli RNase E and RNase G cleave a Bacillus subtilis transcript at the same site in a structure-dependent manner. Arch. Microbiol. 181:137-43
Abstract	The decay of Bacillus subtilis aprE leader- lacZ mRNA was examined in Escherichia coli wild-type and in mutants deficient in RNase E, RNase G, or both. Two versions of the mRNA were studied: the wild-type mRNA, which has a stem-loop at the 5' end, and a mutant mRNA, with a single-stranded 5' end. The half-life of both transcripts was determined by RNase E, the half-life of the mutant transcript being one-third of that of the wild-type transcript. RNase G cleaved both transcripts at a site within an AU-rich sequence in the stem-loop region, but cleavage was much more efficient when the stem-loop was destabilized. RNase E cleaved at the same site, but less efficiently and only in the mutant transcript.
Links	PubMed Online version:10.1007/s00203-003-0637-1
Keywords	Bacillus subtilis/genetics; Bacterial Proteins/genetics; Base Sequence; Endoribonucleases/genetics; Endoribonucleases/metabolism; Escherichia coli/enzymology; Escherichia coli/genetics; Escherichia coli Proteins; Genes, Bacterial; Half-Life; Membrane Transport Proteins/genetics; Molecular Sequence Data; Mutation; Nucleic Acid Conformation; RNA, Bacterial/metabolism; RNA, Messenger/metabolism; Recombinant Fusion Proteins/genetics; Recombinant Fusion Proteins/metabolism; beta-Galactosidase/genetics
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