PMID:14668138

Citation	Plantinga, TH, Van Der Does, C, Badia, J, Aguilar, J, Konings, WN and Driessen, AJ Functional characterization of the Escherichia coli K-12 yiaMNO transport protein genes. Mol. Membr. Biol. 21:51-7
Abstract	The yiaMNO genes of Escherichia coli K-12 encode a binding protein-dependent secondary, or tri-partite ATP-independent periplasmic (TRAP), transporter. Since only a few members of this family have been functionally characterized to date, we aimed to identify the substrate for this transporter. Cells that constitutively express the yiaK-S gene cluster metabolized the rare pentose L-xylulose, while deletion of the yiaMNO transporter genes reduced L-xylulose metabolism. The periplasmic substrate-binding protein YiaO was found to bind L-xylulose, and stimulated L-xylulose uptake by spheroplasts. These date indicate that the yiaMNO transporter mediates uptake of this rare pentose.
Links	PubMed Online version:10.1080/09687680310001607369
Keywords	ATP-Binding Cassette Transporters/metabolism; Base Sequence; Biological Transport/genetics; Carrier Proteins/genetics; Carrier Proteins/metabolism; Chromosomes, Bacterial; Cloning, Molecular; Escherichia coli Proteins/genetics; Gene Expression Regulation, Bacterial; Genes, Bacterial; Membrane Transport Proteins/genetics; Membrane Transport Proteins/metabolism; Molecular Sequence Data; Operon/genetics; Restriction Mapping; Spheroplasts/metabolism; Xylulose/metabolism
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