PMID:14646089
| Citation |
Muranova, TA, Sedelnikova, SE, Leonard, PM, Pasquo, A, Bolt, EL, Lloyd, RG and Rafferty, JB (2003) Crystallization of RusA Holliday junction resolvase from Escherichia coli. Acta Crystallogr. D Biol. Crystallogr. 59:2262-4 |
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| Abstract |
Crystals of the Escherichia coli Holliday junction resolvase RusA have been obtained using the hanging-drop method and characterized. The crystals have a primitive monoclinic form and belong to space group P2(1). The V(M) value suggests the presence of two copies of the monomer in the asymmetric unit. A full three-wavelength MAD data collection on a selenomethionine-incorporated form has been undertaken and structure determination is under way using data collected to 2.1 A resolution. |
| Links | |
| Keywords |
Crystallization/methods; Crystallography, X-Ray/methods; Escherichia coli/enzymology; Escherichia coli Proteins/chemistry; Escherichia coli Proteins/genetics; Holliday Junction Resolvases/chemistry; Holliday Junction Resolvases/genetics; Recombinant Proteins/chemistry; Recombinant Proteins/genetics; Selenomethionine/chemistry |
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