PMID:14640961
| Citation |
Vainonen, YP, Vorobyeva, NN, Kurilova, SA, Nazarova, TI, Rodina, EV and Avaeva, SM (2003) Active dimeric form of inorganic pyrophosphatase from Escherichia coli. Biochemistry Mosc. 68:1195-9 |
|---|---|
| Abstract |
A dimeric form can be obtained from native hexameric Escherichia coli inorganic pyrophosphatase (E-PPase) by destroying the hydrophobic intersubunit contacts, and it has been shown earlier to consist of the subunits of different trimers. The present paper is devoted to the kinetic characterization of such a "double-decked" dimer obtained by the dissociation of either the native enzyme or the mutant variant Glu145Gln. The dimeric form of the native inorganic pyrophosphatase was shown to retain high catalytic efficiency that is in sharp contrast to the dimers obtained as a result of the mutations at the intertrimeric interface. The dimeric enzymes described in the present paper, however, have lost the regulatory properties, in contrast to the hexameric and trimeric forms of the enzyme. |
| Links | |
| Keywords |
Amino Acid Substitution; Catalysis; Cloning, Molecular; Dimerization; Escherichia coli/chemistry; Escherichia coli/enzymology; Escherichia coli/genetics; Inorganic Pyrophosphatase/chemistry; Inorganic Pyrophosphatase/genetics; Inorganic Pyrophosphatase/isolation & purification; Kinetics; Mutagenesis, Site-Directed; Protein Structure, Quaternary/genetics; Recombinant Proteins/chemistry; Recombinant Proteins/genetics; Recombinant Proteins/isolation & purification; Structure-Activity Relationship; Substrate Specificity |
| edit table |
Significance
You can help EcoliWiki by summarizing why this paper is useful
Useful Materials and Methods
You can help Ecoliwiki by describing the useful materials (strains, plasmids, antibodies, etc) described in this paper.
Annotations
<annotationlinks/>
EcoliWiki Links
Add links to pages that link here (e.g. gene, product, method pages)
References
See Help:References for how to manage references in EcoliWiki.