PMID:14633099

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Citation

Li, X and Ricke, SC (2003) Generation of an Escherichia coli lysA targeted deletion mutant by double cross-over recombination for potential use in a bacterial growth-based lysine assay. Lett. Appl. Microbiol. 37:458-62

Abstract

To generate a stable Escherichia coli lysine auxotroph for the lysine bioavailability assay.

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Keywords

Ampicillin Resistance/genetics; Bacterial Proteins; Carboxy-Lyases/genetics; Carboxy-Lyases/metabolism; Escherichia coli/enzymology; Escherichia coli/genetics; Escherichia coli/growth & development; Gene Deletion; Genes, Bacterial; Lysine/biosynthesis; Lysine/genetics; Lysine/isolation & purification; Lysine/metabolism; Mutation/genetics; Recombination, Genetic; Transduction, Genetic; Transformation, Bacterial

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