PMID:14612240
| Citation |
Messenger, SL and Green, J (2003) FNR-mediated regulation of hyp expression in Escherichia coli. FEMS Microbiol. Lett. 228:81-6 |
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| Abstract |
The hypA-E operon is involved in the maturation of all three NiFe hydrogenases in Escherichia coli. Two hyp promoters have been described; a sigma54-dependent promoter upstream of hypA, and a sigma70-dependent promoter (PhypA) within the hypA coding region. Here it is shown that the oxygen-responsive transcription factor FNR regulates PhypA under anaerobic conditions only. PhypA does not possess a canonical FNR recognition sequence, but two FNR half-sites are present. Studies using PHYPA::lacZ fusions carrying lesions in one or both FNR half-sites indicated that although some residual anaerobic activity was retained by the promoter containing only the downstream FNR half-site, both half-sites are required for maximal PhypA activity in vivo. In vitro gel retardation analysis suggested that the primary interaction occurs at the downstream FNR half-site. Possible explanations for these observations and the implications for other FNR-regulated promoters are discussed. |
| Links | |
| Keywords |
Anaerobiosis; Base Sequence; Escherichia coli/genetics; Escherichia coli Proteins/genetics; Escherichia coli Proteins/metabolism; Gene Expression Regulation, Bacterial; Hydrogenase/genetics; Hydrogenase/metabolism; Iron-Sulfur Proteins/genetics; Iron-Sulfur Proteins/metabolism; Molecular Sequence Data; Mutagenesis; Operon; Oxygen/metabolism; Promoter Regions, Genetic; Sigma Factor/metabolism; Transcription Factors/genetics |
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