PMID:1460285
| Citation |
Zezza, DJ, Stewart, SE and Steiner, LA (1992) Genes encoding Xenopus laevis Ig L chains. Implications for the evolution of kappa and lambda chains. J. Immunol. 149:3968-77 |
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| Abstract |
Xenopus laevis Ig contain two distinct types of L chains, designated rho or L1 and sigma or L2. We have analyzed Xenopus genomic DNA by Southern blotting with cDNA probes specific for L1 V and C regions. Many fragments hybridized to the V probe, but only one or two fragments hybridized to the C probe. Corresponding C, J, and V gene segments were identified on clones isolated from a genomic library prepared from the same DNA. One clone contains a C gene segment separated from a J gene segment by an intron of 3.4 kb. The J and C gene segments are nearly identical in sequence to cDNA clones analyzed previously. The C segment is somewhat more similar and the J segment considerably more similar in sequence to the corresponding segments of mammalian kappa chains than to those of mammalian lambda chains. Upstream of the J segment is a typical recombination signal sequence with a spacer of 23 bp, as in J kappa. A second clone from the library contains four V gene segments, separated by 2.1 to 3.6 kb. Two of these, V1 and V3, have the expected structural and regulatory features of V genes, and are very similar in sequence to each other and to mammalian V kappa. A third gene segment, V2, resembles V1 and V3 in its coding region and nearby 5'-flanking region, but diverges in sequence 5' to position -95 with loss of the octamer promoter element. The fourth V-like segment is similar to the others at the 3'-end, but upstream of codon 64 bears no resemblance in sequence to any Ig V region. All four V segments have typical recombination signal sequences with 12-bp spacers at their 3'-ends, as in V kappa. Taken together, the data suggest that Xenopus L1 L chain genes are members of the kappa gene family. |
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| Keywords |
Amino Acid Sequence; Animals; Base Sequence; Blotting, Southern; DNA/analysis; Gene Library; Genes, Immunoglobulin; Immunoglobulin Constant Regions/genetics; Immunoglobulin Joining Region/genetics; Immunoglobulin Light Chains/genetics; Immunoglobulin Variable Region/genetics; Immunoglobulin kappa-Chains/genetics; Molecular Sequence Data; Phylogeny; Restriction Mapping; Sequence Alignment; Sequence Analysis, DNA; Sequence Homology, Amino Acid; Sequence Homology, Nucleic Acid; Xenopus laevis/genetics |
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