PMID:1459954
| Citation |
Muda, M, Rao, NN and Torriani, A (1992) Role of PhoU in phosphate transport and alkaline phosphatase regulation. J. Bacteriol. 174:8057-64 |
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| Abstract |
The negative regulatory function of PhoU in alkaline phosphatase (AP) was suggested by the behavior of K10 phoU35 carrying a missense mutation whose product was detected by immunoblotting. To define more clearly the regulatory function of this protein for the synthesis of AP, we constructed a null mutation. The constitutive synthesis of AP in this phoU deletion strain confirmed the negative role of PhoU. However, the expression of the PhoU protein from an isopropyl-beta-D-thiogalactopyranoside-inducible promoter had no effect on the repression of AP synthesis. Furthermore, the involvement of PhoU in free-Pi uptake was demonstrated. These results provide evidence that PhoU participates in Pi transport and in the regulatory role of the phosphate-specific transport system. |
| Links | |
| Keywords |
Alkaline Phosphatase/biosynthesis; Bacterial Proteins/genetics; Bacterial Proteins/metabolism; Biological Transport/genetics; Blotting, Southern; Chromosome Deletion; Cloning, Molecular; Electrophoresis, Polyacrylamide Gel; Enzyme Induction/genetics; Escherichia coli Proteins; Genetic Complementation Test; Immunoblotting; Kinetics; Membrane Transport Proteins; Phosphates/metabolism; Plasmids; Transcription Factors |
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