PMID:14597009
| Citation |
Kusano, K, Asami, Y, Fujita, A, Tanokura, M and Kobayashi, I (2003) Type I restriction enzyme with RecA protein promotes illegitimate recombination. Plasmid 50:202-12 |
|---|---|
| Abstract |
Illegitimate (non-homologous) recombination requires little or no sequence homology between recombining DNAs and has been regarded as being a process distinct from homologous recombination, which requires a long stretch of homology between recombining DNAs. However, we have found a type of illegitimate recombination that requires an interaction between long homologous DNA sequences. It was detected when a plasmid that carried 2-kb-long inverted repeats was subjected to type I (EcoKI) restriction in vivo within a special mutant strain of Escherichia coli. In the present work, we analyzed genetic requirements for this type of illegitimate recombination in well-defined genetic backgrounds. Our analysis demonstrated dependence on RecA function and on the presence of two EcoKI sites on the substrate DNA. These results are in harmony with a model in which EcoKI restriction enzyme attacks an intermediate of homologous recombination to divert it to illegitimate recombination. |
| Links | |
| Keywords |
Base Sequence; DNA Restriction Enzymes/genetics; DNA Restriction Enzymes/metabolism; Deoxyribonucleases, Type I Site-Specific/genetics; Deoxyribonucleases, Type I Site-Specific/metabolism; Escherichia coli/genetics; Escherichia coli Proteins/genetics; Escherichia coli Proteins/metabolism; Models, Genetic; Molecular Sequence Data; Plasmids/genetics; Rec A Recombinases/genetics; Rec A Recombinases/metabolism; Recombination, Genetic; Repetitive Sequences, Nucleic Acid; Sequence Homology, Nucleic Acid |
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