PMID:14596903

Citation	Ehrle, R, Mikhaleva, N, Boyd, D, Davidson, AL and Ehrmann, M (2003) Context-dependent effects of charged residues in transmembrane segments of MalF-PhoA fusions. Res. Microbiol. 154:654-7
Abstract	Charged residues were introduced into the C-terminal transmembrane segment of a MalF-PhoA fusion to investigate the efficiency of the altered transmembrane segment to function as an export signal or a stop transfer signal. PhoA assays revealed that charges had negative effects when the transmembrane segment was part of a stop transfer signal but not when it was part of an export signal. Implications of this finding for the biogenesis of polytopic membrane proteins are discussed.
Links	PubMed Online version:10.1016/j.resmic.2003.07.001
Keywords	ATP-Binding Cassette Transporters/metabolism; Alkaline Phosphatase; Biological Transport; Carrier Proteins/chemistry; Carrier Proteins/genetics; Carrier Proteins/metabolism; Cyclin-Dependent Kinases/chemistry; Cyclin-Dependent Kinases/genetics; Cyclin-Dependent Kinases/metabolism; Escherichia coli/metabolism; Escherichia coli Proteins/metabolism; Maltose-Binding Proteins; Membrane Proteins/chemistry; Membrane Proteins/metabolism; Models, Biological; Monosaccharide Transport Proteins/metabolism
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