PMID:14595014
| Citation |
Neher, SB, Sauer, RT and Baker, TA (2003) Distinct peptide signals in the UmuD and UmuD' subunits of UmuD/D' mediate tethering and substrate processing by the ClpXP protease. Proc. Natl. Acad. Sci. U.S.A. 100:13219-24 |
|---|---|
| Abstract |
The Escherichia coli UmuD' protein is a component of DNA polymerase V, an error-prone polymerase that carries out translesion synthesis on damaged DNA templates. The intracellular concentration of UmuD' is strictly controlled by regulated transcription, by posttranslational processing of UmuD to UmuD', and by ClpXP degradation. UmuD' is a substrate for the ClpXP protease but must form a heterodimer with its unabbreviated precursor, UmuD, for efficient degradation to occur. Here, we show that UmuD functions as a UmuD' delivery protein for ClpXP. UmuD can also deliver a UmuD partner for degradation. UmuD resembles SspB, a well-characterized substrate-delivery protein for ClpX, in that both proteins use related peptide motifs to bind to the N-terminal domain of ClpX, thereby tethering substrate complexes to ClpXP. The combined use of a weak substrate recognition signal and a delivery factor that tethers the substrate to the protease allows regulated proteolysis of UmuD/D' in the cell. Dual recognition strategies of this type may be a relatively common feature of intracellular protein turnover. |
| Links |
PubMed PMC263758 Online version:10.1073/pnas.2235804100 |
| Keywords |
Adenosine Triphosphatases/metabolism; Amino Acid Sequence; Bacterial Proteins/chemistry; Bacterial Proteins/metabolism; DNA Damage; DNA Repair; DNA-Directed DNA Polymerase; Dimerization; Dose-Response Relationship, Drug; Endopeptidase Clp; Escherichia coli Proteins/chemistry; Escherichia coli Proteins/metabolism; Kinetics; Models, Biological; Molecular Sequence Data; Peptides/chemistry; Protein Binding; Protein Processing, Post-Translational; Protein Structure, Tertiary; Sequence Homology, Amino Acid; Serine Endopeptidases/metabolism; Time Factors |
| edit table |
Significance
You can help EcoliWiki by summarizing why this paper is useful
Useful Materials and Methods
You can help Ecoliwiki by describing the useful materials (strains, plasmids, antibodies, etc) described in this paper.
Annotations
<annotationlinks/>
EcoliWiki Links
Add links to pages that link here (e.g. gene, product, method pages)
References
See Help:References for how to manage references in EcoliWiki.