PMID:14578344

Citation	van der Laan, M, Nouwen, N and Driessen, AJ (2004) SecYEG proteoliposomes catalyze the Deltaphi-dependent membrane insertion of FtsQ. J. Biol. Chem. 279:1659-64
Abstract	In Escherichia coli, the insertion of most inner membrane proteins is mediated by the Sec translocase. Ribosome-bound nascent chains of Sec-dependent inner membrane proteins are targeted to the SecYEG complex via the signal recognition particle pathway. We now demonstrate that the signal recognition particle-dependent co-translational membrane targeting and membrane insertion of FtsQ can be reconstituted with proteoliposomes containing purified SecYEG. SecA and a transmembrane electrical potential are essential for the translocation of the large periplasmic domain of FtsQ, whereas co-reconstituted YidC has an inhibitory effect. These data demonstrate that membrane protein insertion can be reconstituted with a minimal set of purified Sec components.
Links	PubMed Online version:10.1074/jbc.M306527200
Keywords	Adenosine Triphosphatases/metabolism; Bacterial Proteins/metabolism; Catalysis; Escherichia coli Proteins/metabolism; Escherichia coli Proteins/physiology; Membrane Proteins/metabolism; Membrane Transport Proteins/metabolism; Membrane Transport Proteins/physiology; Proteolipids/physiology; Proton-Motive Force
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