PMID:14572658

Citation	Kuo, JT, Chang, YJ and Tseng, CP (2003) Growth rate regulation of lac operon expression in Escherichia coli is cyclic AMP dependent. FEBS Lett. 553:397-402
Abstract	In contrast to the ribosomal RNA gene expression increasing with growth rate, transcription of the lac operon is downregulated by cell growth rate. In continuous culture, growth rate regulation of lac promoter was independent of carbon substrate used and its location on the chromosome. Since the lac operon is activated by cyclic adenosine monophosphate (cAMP), which decreases with increasing cell growth rate, expression of plac-lacZ reporter fusion was analyzed in cya mutant under various growth conditions. The results demonstrated that expression of plac-lacZ in cya mutant was both lower and growth rate independent. In addition, ppGpp (guanosine tetraphosphate) was not involved in the mechanism of growth rate regulation of the lac promoter. Thus, the results of this study indicate that cAMP mediates the growth rate-dependent regulation of lac operon expression in Escherichia coli.
Links	PubMed
Keywords	Bacteriophages/genetics; Bacteriophages/growth & development; Bacteriophages/metabolism; Carbon/metabolism; Cyclic AMP/metabolism; Cyclic AMP Receptor Protein/genetics; Escherichia coli/genetics; Escherichia coli/growth & development; Escherichia coli/metabolism; Gene Deletion; Gene Expression Regulation/genetics; Genotype; Guanosine Tetraphosphate/metabolism; Lac Operon/physiology; Mutation; Phenotype; Plasmids/genetics; Species Specificity; Transcription, Genetic; beta-Galactosidase/metabolism
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