PMID:14517231

Citation	Bernstein, DA, Zittel, MC and Keck, JL (2003) High-resolution structure of the E.coli RecQ helicase catalytic core. EMBO J. 22:4910-21
Abstract	RecQ family helicases catalyze critical genome maintenance reactions in bacterial and eukaryotic cells, playing key roles in several DNA metabolic processes. Mutations in recQ genes are linked to genome instability and human disease. To define the physical basis of RecQ enzyme function, we have determined a 1.8 A resolution crystal structure of the catalytic core of Escherichia coli RecQ in its unbound form and a 2.5 A resolution structure of the core bound to the ATP analog ATPgammaS. The RecQ core comprises four conserved subdomains; two of these combine to form its helicase region, while the others form unexpected Zn(2+)-binding and winged-helix motifs. The structures reveal the molecular basis of missense mutations that cause Bloom's syndrome, a human RecQ-associated disease. Finally, based on findings from the structures, we propose a mechanism for RecQ activity that could explain its functional coordination with topoisomerase III.
Links	PubMed PMC204483 Online version:10.1093/emboj/cdg500
Keywords	Adenosine Triphosphatases/chemistry; Adenosine Triphosphatases/genetics; Amino Acid Sequence; Catalytic Domain; DNA Helicases/chemistry; DNA Helicases/genetics; Escherichia coli/enzymology; Escherichia coli/genetics; Molecular Sequence Data; Multigene Family/genetics; Protein Structure, Tertiary; RecQ Helicases
edit table

Significance

You can help EcoliWiki by summarizing why this paper is useful

You can help Ecoliwiki by describing the useful materials (strains, plasmids, antibodies, etc) described in this paper.

Add links to pages that link here (e.g. gene, product, method pages)

See Help:References for how to manage references in EcoliWiki.