PMID:14514676
| Citation |
Mouslim, C, Latifi, T and Groisman, EA (2003) Signal-dependent requirement for the co-activator protein RcsA in transcription of the RcsB-regulated ugd gene. J. Biol. Chem. 278:50588-95 |
|---|---|
| Abstract |
The RcsC/YojN/RcsB phosphorelay system controls gene expression in response to a variety of signals, including changes in temperature, osmolarity, and overproduction of membrane proteins. Transcription of certain RcsB-activated genes, such as the capsule synthesis cps operon, requires the co-activator protein RcsA, whereas expression of other RcsB-activated genes is RcsA-independent. We have established previously that a tolB mutation induces transcription of the Salmonella UDP-glucose dehydrogenase ugd gene in an RcsA- and RcsB-dependent manner. This induction is independent of the two-component systems PhoP/PhoQ and PmrA/PmrB, which are required for ugd expression in response to low Mg2+. We now report that the RcsC/YojN/RcsB system is activated in a pmrA mutant experiencing Fe3+ and low Mg2+, resulting in expression of both cps and ugd genes. However, whereas cps transcription remained RcsA-dependent, ugd transcription became RcsA-independent but dependent on the PhoP protein. S1 mapping experiments demonstrated that RcsA-dependent and -independent transcription of the ugd gene use the same promoter. DNase footprinting analysis identified a PhoP-binding site in the ugd promoter. Yet, PhoP-mediated ugd transcription required either the RcsC/YojN/RcsB or the PmrA/PmrB systems. |
| Links |
PubMed Online version:10.1074/jbc.M309433200 |
| Keywords |
Bacterial Proteins/metabolism; Base Sequence; Binding Sites; Chromosomes/ultrastructure; Deoxyribonuclease I/metabolism; Escherichia coli Proteins/chemistry; Escherichia coli Proteins/metabolism; Gene Deletion; Iron/metabolism; Magnesium/metabolism; Models, Biological; Models, Genetic; Molecular Sequence Data; Mutation; Periplasmic Proteins/metabolism; Plasmids/metabolism; Promoter Regions, Genetic; Salmonella/metabolism; Sequence Homology, Amino Acid; Single-Strand Specific DNA and RNA Endonucleases/metabolism; Temperature; Transcription Factors; Transcription, Genetic; beta-Galactosidase/metabolism |
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