PMID:14504292

Citation	Illarionov, B, Haase, I, Bacher, A, Fischer, M and Schramek, N (2003) Presteady state kinetic analysis of riboflavin synthase. J. Biol. Chem. 278:47700-6
Abstract	Riboflavin synthase catalyzes a mechanistically complex dismutation affording riboflavin and 5-amino-6-ribitylamino-2,4(1H,3H)-pyrimidinedione from 6,7-dimethyl-8-ribityllumazine. The kinetics of the enzyme from Escherichia coli were studied under single turnover conditions. Stopped flow as well as quenched flow experiments documented the transient formation of a pentacyclic reaction intermediate. No other transient species were sufficiently populated to allow detection. The data are best described by a sequence of one second order and one first order reaction.
Links	PubMed Online version:10.1074/jbc.M305050200
Keywords	Catalysis; Chromatography, High Pressure Liquid; Crystallography, X-Ray; Escherichia coli/enzymology; Hydrogen-Ion Concentration; Kinetics; Models, Chemical; Riboflavin/chemistry; Riboflavin Synthase/chemistry; Time Factors
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