PMID:1447129

Citation	Baril, C, Herrmann, JL, Richaud, C, Margarita, D and Girons, IS (1992) Scattering of the rRNA genes on the physical map of the circular chromosome of Leptospira interrogans serovar icterohaemorrhagiae. J. Bacteriol. 174:7566-71
Abstract	Leptospira interrogans is a pathogenic bacterium with a low G+C content (34 to 39%). The restriction enzymes NotI, AscI, and SrfI cut the chromosome of L. interrogans serovar icterohaemorrhagiae into 13, 3, and 5 fragments separable by one- and two-dimensional pulsed-field gel electrophoresis (PFGE). The genome is composed of a circular 4.6-Mbp chromosome and a 0.35-Mbp extrachromosomal element. A physical map of the chromosome was constructed for NotI, AscI, and SrfI by using single and double digests, or partial NotI digests obtained at random or by cross-protection of NotI sites by FnuDII methylase, and linking clones. rRNA genes were found to be widely scattered on the chromosome.
Links	PubMed PMC207466
Keywords	Base Sequence; Chromosome Mapping; Chromosomes, Bacterial; Cloning, Molecular; Deoxyribonucleases, Type II Site-Specific/metabolism; Genes, Bacterial/genetics; Genome, Bacterial; Leptospira interrogans/genetics; Molecular Sequence Data; RNA, Ribosomal/genetics; Serotyping
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