PMID:1435256
| Citation |
Benhar, I, Miller, C and Engelberg-Kulka, H (1992) Frameshifting in the expression of the Escherichia coli trpR gene. Mol. Microbiol. 6:2777-84 |
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| Abstract |
The trpR gene of Escherichia coli carries an open reading frame that encodes the trp repressor, 108 amino acids long. Here we show that translation of an additional (+1) reading frame of trpR occurs both in vivo and in vitro. This results in the synthesis of a stable +1 frame polypeptide. Using site-specific mutagenesis, immunological techniques and amino acid sequencing we have found that the N-terminus of the +1 frame product and that of the known 0 frame product are identical but that their C-termini differ. Our results are discussed in relation to the role of natural frameshifting as a regulatory mechanism of gene expression in general, and with respect to tryptophan biosynthesis in particular. |
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| Keywords |
Amino Acid Sequence; Bacterial Proteins/biosynthesis; Bacterial Proteins/genetics; Base Sequence; Codon; Escherichia coli/genetics; Escherichia coli/metabolism; Gene Expression Regulation, Bacterial; Molecular Sequence Data; Mutagenesis, Site-Directed; Open Reading Frames; Protein Biosynthesis; Repressor Proteins/biosynthesis; Repressor Proteins/genetics; Tryptophan/genetics |
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