PMID:1409640
| Citation |
Matijasevic, Z, Sekiguchi, M and Ludlum, DB (1992) Release of N2,3-ethenoguanine from chloroacetaldehyde-treated DNA by Escherichia coli 3-methyladenine DNA glycosylase II. Proc. Natl. Acad. Sci. U.S.A. 89:9331-4 |
|---|---|
| Abstract |
The human carcinogen vinyl chloride is metabolized in the liver to reactive intermediates which form N2,3-ethenoguanine in DNA. N2,3-Ethenoguanine is known to cause G----A transitions during DNA replication in Escherichia coli, and its formation may be a carcinogenic event in higher organisms. To investigate the repair of N2,3-ethenoguanine, we have prepared an N2,3-etheno[14C]guanine-containing DNA substrate by nick-translating DNA with [14C]dGTP and modifying the product with chloroacetaldehyde. E. coli 3-methyladenine DNA glycosylase II, purified from cells which carry the plasmid pYN1000, releases N2,3-ethenoguanine from chloroacetaldehyde-modified DNA in a protein- and time-dependent manner. This finding widens the known substrate specificity of glycosylase II to include a modified base which may be associated with the carcinogenic process. Similar enzymatic activity in eukaryotic cell might protect them from exposure to metabolites of vinyl chloride. |
| Links | |
| Keywords |
Acetaldehyde/analogs & derivatives; Acetaldehyde/pharmacology; Carbon Radioisotopes; Chromatography, High Pressure Liquid; DNA/drug effects; DNA/metabolism; DNA Glycosylases; DNA Repair; Deoxyguanine Nucleotides/metabolism; Escherichia coli/enzymology; Guanine/analogs & derivatives; Guanine/analysis; Kinetics; Methylnitrosourea/metabolism; N-Glycosyl Hydrolases/isolation & purification; N-Glycosyl Hydrolases/metabolism; Time Factors; Tritium |
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