PMID:1400180
| Citation |
Garzón, A, Li, J, Flores, A, Casadesus, J and Stewart, V (1992) Molybdenum cofactor (chlorate-resistant) mutants of Klebsiella pneumoniae M5al can use hypoxanthine as the sole nitrogen source. J. Bacteriol. 174:6298-302 |
|---|---|
| Abstract |
Selection for chlorate resistance yields mol (formerly chl) mutants with defects in molybdenum cofactor synthesis. Complementation and genetic mapping analyses indicated that the Klebsiella pneumoniae mol genes are functionally homologous to those of Escherichia coli and occupy analogous genetic map positions. Hypoxanthine utilization in other organisms requires molybdenum cofactor as a component of xanthine dehydrogenase, and thus most chlorate-resistant mutants cannot use hypoxanthine as a sole source of nitrogen. Surprisingly, the K. pneumoniae mol mutants and the mol+ parent grew equally well with hypoxanthine as the sole nitrogen source, suggesting that K. pneumoniae has a molybdenum cofactor-independent pathway for hypoxanthine utilization. |
| Links | |
| Keywords |
Chromosome Mapping; Coenzymes/genetics; Gene Expression Regulation, Bacterial; Genetic Complementation Test; Hypoxanthine; Hypoxanthines/metabolism; Klebsiella pneumoniae/genetics; Klebsiella pneumoniae/metabolism; Metalloproteins/metabolism; Molybdenum/metabolism; Mutagenesis; Nitrate Reductase; Nitrate Reductases/analysis; Nitrogen/metabolism; Pteridines/metabolism; Transduction, Genetic; Tungsten/pharmacology; Tungsten Compounds |
| edit table |
Significance
You can help EcoliWiki by summarizing why this paper is useful
Useful Materials and Methods
You can help Ecoliwiki by describing the useful materials (strains, plasmids, antibodies, etc) described in this paper.
Annotations
<annotationlinks/>
EcoliWiki Links
Add links to pages that link here (e.g. gene, product, method pages)
References
See Help:References for how to manage references in EcoliWiki.