PMID:1397315
| Citation |
Brandt, R and Gualerzi, CO (1992) Ribosomal localization of the mRNA in the 30S initiation complex as revealed by UV crosslinking. FEBS Lett. 311:199-202 |
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| Abstract |
Translation initiation complexes consisting of 30S ribosomal subunits, 32P-labelled mRNA (002 mRNA), fMet-tRNA and the three initiation factors were subjected to UV-crosslinking to determine the protein and rRNA neighbors of the bound mRNA by immunochemical methods and by nucleic acid hybridization techniques. The mRNA was found to be crosslinked to a specific region of the 16S rRNA spanning from nucleotide 418 to 615 and to ribosomal proteins S1 and S21 (the main targets), S3, S10, S12 and S14; a low level of crosslinking was also detected with S2, S7, S13, S18 and S19. |
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| Keywords |
Base Sequence; Computer Simulation; Cross-Linking Reagents; Escherichia coli/metabolism; Immunoblotting; Macromolecular Substances; Models, Structural; Molecular Sequence Data; Peptide Chain Initiation, Translational; Peptide Initiation Factors/analysis; Peptide Initiation Factors/metabolism; RNA, Messenger/analysis; RNA, Messenger/genetics; RNA, Messenger/metabolism; Ribosomal Proteins/analysis; Ribosomal Proteins/metabolism; Ribosomes/metabolism; Ultraviolet Rays |
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