PMID:1386361
| Citation |
Rahman, MA, Nelson, H, Weissbach, H and Brot, N (1992) Cloning, sequencing, and expression of the Escherichia coli peptide methionine sulfoxide reductase gene. J. Biol. Chem. 267:15549-51 |
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| Abstract |
The gene encoding peptide methionine sulfoxide reductase was cloned from an Escherichia coli genomic library using an oligonucleotide probe based on the amino-terminal sequence of the protein. The nucleotide sequence revealed that the gene codes for a polypeptide of 212 amino acid residues with a calculated molecular weight of 23,314. The protein has been overexpressed in E. coli and is present as a soluble active species. |
| Links | |
| Keywords |
Amino Acid Sequence; Bacteriophage lambda/genetics; Base Sequence; Cloning, Molecular; DNA Probes; Escherichia coli/enzymology; Escherichia coli/genetics; Genes, Bacterial; Genomic Library; Kinetics; Methionine Sulfoxide Reductases; Molecular Sequence Data; Oligonucleotide Probes; Oxidoreductases/genetics; Oxidoreductases/metabolism; Recombinant Proteins/metabolism |
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