PMID:1339356
| Citation |
Magnuson, K, Oh, W, Larson, TJ and Cronan, JE Jr (1992) Cloning and nucleotide sequence of the fabD gene encoding malonyl coenzyme A-acyl carrier protein transacylase of Escherichia coli. FEBS Lett. 299:262-6 |
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| Abstract |
We report the cloning and nucleotide sequence of the gene encoding malonyl coenzyme A-acyl carrier protein transacylase of Escherichia coli. Malonyl transacylase has been overexpressed 155-fold compared to a wild-type strain. Overexpression of this enzyme alters the fatty acid composition of a wild-type E. coli strain; increased amounts of cis-vaccenate are incorporated into the membrane phospholipids. |
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| Keywords |
Acyl-Carrier Protein S-Malonyltransferase; Acyltransferases/genetics; Amino Acid Sequence; Base Sequence; Cloning, Molecular; Enzyme Induction; Escherichia coli/genetics; Escherichia coli Proteins; Fatty Acid Synthetase Complex, Type II; Fatty Acids/analysis; Gene Expression Regulation, Bacterial; Membranes/chemistry; Molecular Sequence Data; Phospholipids/chemistry; Sequence Homology, Nucleic Acid |
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