PMID:1335453
| Citation |
Reid, MB, Shoji, T, Moody, MR and Entman, ML (1992) Reactive oxygen in skeletal muscle. II. Extracellular release of free radicals. J. Appl. Physiol. 73:1805-9 |
|---|---|
| Abstract |
We have tested the hypothesis that diaphragm muscle fibers release superoxide anion radicals (O2-.) into the extracellular space. Fiber bundles were isolated from rat diaphragm and incubated in Krebs-Ringer solution containing cytochrome c (10(-5) M), a standard assay for O2-.. Bundles were either passive or active, i.e., directly stimulated to contract rhythmically. After 1 h, absorbance of reduced cytochrome c in the incubation medium was measured at 550 nm. Absorbance was greater in medium exposed to passive muscle than in medium without muscle (P < 0.01), indicating O2-. release by passive muscle. Absorbance was greater in medium exposed to active muscle than in that exposed to passive muscle (P < 0.01), an increase inhibited by superoxide dismutase (10(3) U/ml). Active bundles fatigued; bundles developing the lowest final stresses produced the greatest absorbance increases (P < 0.001), suggesting that the magnitude of fatigue was inversely related to O2-. release. We conclude that O2-. is released by diaphragm myocytes into the interstitium and surrounding medium, a process accelerated by fatiguing muscular contractions. |
| Links | |
| Keywords |
Animals; Cytochrome c Group/metabolism; Electric Stimulation; Extracellular Space/metabolism; Free Radicals/metabolism; Male; Muscle Contraction/physiology; Muscles/metabolism; Rats; Rats, Sprague-Dawley; Reactive Oxygen Species/metabolism; Respiratory Muscles/physiology; Xanthine Oxidase/metabolism |
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