PMID:1326408
| Citation |
Gegner, JA, Graham, DR, Roth, AF and Dahlquist, FW (1992) Assembly of an MCP receptor, CheW, and kinase CheA complex in the bacterial chemotaxis signal transduction pathway. Cell 70:975-82 |
|---|---|
| Abstract |
We examined the binding interactions of the methylation-dependent chemotaxis receptors Tsr and Tar with the chemotaxis-specific protein kinase CheA and the coupling factor CheW. Receptor directly bound CheW, but receptor-CheA binding was dependent upon the presence of CheW. These observations in combination with our previous identification of a CheW-CheA complex suggest that CheW physically links the kinase to the receptor. The ternary complex of receptor, CheW, and CheA is both kinetically and thermodynamically stable at physiological concentrations. Stability is not significantly altered by changes associated with attractant or repellent binding to the receptor. Such binding greatly modulates the kinase activity of CheA. Our results demonstrate that modulation of the kinase activity does not require association-dissociation of the ternary complex. This suggests that the receptor signal is transduced through conformational changes in the ternary complex rather than through changes in the association of the kinase CheA with receptor and/or CheW. |
| Links | |
| Keywords |
Bacterial Proteins/metabolism; Chemoreceptor Cells; Chemotactic Factors/metabolism; Chemotaxis/physiology; Escherichia coli Proteins; Kinetics; Membrane Proteins/metabolism; Protein Binding; Receptors, Cell Surface/metabolism; Signal Transduction/physiology |
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