PMID:1320612
| Citation |
Meyer, CR, Ghosh, P, Remy, E and Preiss, J (1992) Cloning, expression, and nucleotide sequence of a mutant glgC gene from Escherichia coli B. J. Bacteriol. 174:4509-12 |
|---|---|
| Abstract |
A mutant glgC gene contained in a 10.9-kb PstI fragment was cloned from the Escherichia coli B strain SG5 via colony hybridization by using a wild-type glgC probe. The altered allosteric properties of the expressed ADPglucose synthetase were found to result from the conversion of proline to serine at amino acid residue 295. |
| Links | |
| Keywords |
Amino Acid Sequence; Base Sequence; Cloning, Molecular; Escherichia coli/enzymology; Escherichia coli/genetics; Gene Expression; Genes, Bacterial; Glucose-1-Phosphate Adenylyltransferase; Kinetics; Molecular Sequence Data; Mutation; Nucleotidyltransferases/genetics; Nucleotidyltransferases/metabolism; Plasmids; Restriction Mapping |
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