PMID:1316900
| Citation |
Leyh, TS, Vogt, TF and Suo, Y (1992) The DNA sequence of the sulfate activation locus from Escherichia coli K-12. J. Biol. Chem. 267:10405-10 |
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| Abstract |
The DNA sequence of the sulfate activation locus from Escherichia coli K-12 has been determined. The sequence includes the structural genes encoding the enzymes ATP sulfurylase (cysD and cysN) and APS kinase (cysC) which catalyze the synthesis of activated sulfate. These are the only genes known to reside in the sulfate activation operon. Consensus elements of the operon promoter were identified, and the start codons and open reading frames of the Cys polypeptides were determined. During this work, another gene, iap, was partially sequenced and mapped. The activity of ATP sulfurylase is stimulated by an intrinsic GTPase. Comparison of the primary sequences of CysN and Ef-Tu revealed that CysN has conserved many of the residues integral to the three-dimensional structure important for guanine nucleotide binding in Ef-Tu and RAS. nodP and nodQ, from Rhizobium meliloti, are essential for nodulation in leguminous plants. The Cys and Nod proteins are remarkably similar. NodP appears to be the smaller subunit of ATP sulfurylase. NodQ encodes homologues of both CysN and CysC; thus, these enzymes may be covalently associated in R. meliloti. The consensus GTP-binding sequences of NodQ and CysN are identical suggesting that NodQ encodes a regulatory GTPase. |
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| Keywords |
Amino Acid Sequence; Base Sequence; Chromosome Mapping; Codon; DNA, Bacterial/genetics; Escherichia coli/genetics; Escherichia coli/metabolism; Genes, Bacterial; Molecular Sequence Data; Open Reading Frames; Operon; Peptide Elongation Factor Tu/genetics; Phosphotransferases/genetics; Phosphotransferases (Alcohol Group Acceptor); Promoter Regions, Genetic; Protein Biosynthesis; Restriction Mapping; Sequence Alignment; Sulfate Adenylyltransferase/genetics; Sulfates/metabolism; Terminator Regions, Genetic |
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