PMID:13129944
| Citation |
Hagiwara, D, Sugiura, M, Oshima, T, Mori, H, Aiba, H, Yamashino, T and Mizuno, T (2003) Genome-wide analyses revealing a signaling network of the RcsC-YojN-RcsB phosphorelay system in Escherichia coli. J. Bacteriol. 185:5735-46 |
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| Abstract |
In Escherichia coli, capsular colanic acid polysaccharide synthesis is regulated through the multistep RcsC-->YojN-->RcsB phosphorelay. By monitoring a hallmarked cps::lacZ reporter gene, we first searched for physiological stimuli that propagate the Rcs signaling system. The expression of cps::lacZ was activated when cells were grown at a low temperature (20 degrees C) in the presence of glucose as a carbon source and in the presence of a relatively high concentration of external zinc (1 mM ZnCl(2)). In this Rcs signaling system, the rcsF gene product (a putative outer membrane-located lipoprotein) was also an essential signaling component. Based on the defined signaling pathway and physiological stimuli for the Rcs signaling system, we conducted genome-wide analyses with microarrays to clarify the Rcs transcriptome (i.e., Rcs regulon). Thirty-two genes were identified as putative Rcs regulon members; these genes included 15 new genes in addition to 17 of the previously described cps genes. Using a set of 37 two-component system mutants, we performed alternative genome-wide analyses. The results showed that the propagation of the zinc-responsive Rcs signaling system was largely dependent on another two-component system, PhoQ/P. Considering the fact that the PhoQ/P signaling system responds to external magnesium, we obtained evidence which supports the view that there is a signaling network that connects the Rcs system with the PhoQ/P system, which coordinately regulates extracellular polysaccharide synthesis in response to the external concentrations of divalent cations. |
| Links | |
| Keywords |
Bacterial Capsules/metabolism; Bacterial Proteins/genetics; Bacterial Proteins/metabolism; Base Sequence; Escherichia coli/genetics; Escherichia coli/metabolism; Escherichia coli Proteins/genetics; Escherichia coli Proteins/metabolism; Gene Expression Regulation, Bacterial; Genome, Bacterial; Molecular Sequence Data; Multienzyme Complexes/genetics; Multienzyme Complexes/metabolism; Oligonucleotide Array Sequence Analysis/methods; Phosphoprotein Phosphatases/genetics; Phosphoprotein Phosphatases/metabolism; Phosphotransferases/genetics; Phosphotransferases/metabolism; Polysaccharides/metabolism; Protein Kinases/genetics; Protein Kinases/metabolism; Signal Transduction; Transcription Factors; Zinc/pharmacology |
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