From EcoliWiki
Jump to: navigation, search

Gerdes, SY, Scholle, MD, Campbell, JW, Balázsi, G, Ravasz, E, Daugherty, MD, Somera, AL, Kyrpides, NC, Anderson, I, Gelfand, MS, Bhattacharya, A, Kapatral, V, D'Souza, M, Baev, MV, Grechkin, Y, Mseeh, F, Fonstein, MY, Overbeek, R, Barabási, AL, Oltvai, ZN and Osterman, AL (2003) Experimental determination and system level analysis of essential genes in Escherichia coli MG1655. J. Bacteriol. 185:5673-84


Defining the gene products that play an essential role in an organism's functional repertoire is vital to understanding the system level organization of living cells. We used a genetic footprinting technique for a genome-wide assessment of genes required for robust aerobic growth of Escherichia coli in rich media. We identified 620 genes as essential and 3,126 genes as dispensable for growth under these conditions. Functional context analysis of these data allows individual functional assignments to be refined. Evolutionary context analysis demonstrates a significant tendency of essential E. coli genes to be preserved throughout the bacterial kingdom. Projection of these data over metabolic subsystems reveals topologic modules with essential and evolutionarily preserved enzymes with reduced capacity for error tolerance.


PubMed PMC193955


Aerobiosis; Amino Acids/biosynthesis; Culture Media; DNA Footprinting/methods; DNA Transposable Elements; Escherichia coli/genetics; Escherichia coli/growth & development; Escherichia coli Proteins/genetics; Escherichia coli Proteins/metabolism; Evolution, Molecular; Gene Expression Regulation, Bacterial; Genes, Essential; Genome, Bacterial; Mutagenesis, Insertional; Phylogeny


You can help EcoliWiki by summarizing why this paper is useful

Useful Materials and Methods

You can help Ecoliwiki by describing the useful materials (strains, plasmids, antibodies, etc) described in this paper.



EcoliWiki Links

Add links to pages that link here (e.g. gene, product, method pages)

See also


See Help:References for how to manage references in EcoliWiki.