PMID:1312983
| Citation |
Zheng, L, Wang, X and Braymer, HD (1992) Purification and N-terminal amino acid sequences of two polypeptides encoded by the mcrB gene from Escherichia coli K-12. Gene 112:97-100 |
|---|---|
| Abstract |
This report provides a purification method for the two proteins, 51 kDa and 33 kDa, both encoded by the same mcrB gene of the McrBC restriction system in Escherichia coli K-12. The two proteins were produced in large quantity using a T7 expression system and copurified to near homogeneity by DEAE-Sepharose and Affi-Gel blue column chromatography. The N-terminal amino acid sequences of these purified McrB proteins were the same as those predicted from the mcrB DNA sequence by Ross et al. [J. Bacteriol. 171 (1989b) 1974-1981]. The 33-kDa protein totally overlaps the C-terminal part of the 51-kDa protein. |
| Links | |
| Keywords |
Amino Acid Sequence; Chromatography; DNA Restriction Enzymes/chemistry; DNA Restriction Enzymes/genetics; DNA Restriction Enzymes/isolation & purification; DNA-Cytosine Methylases/chemistry; DNA-Cytosine Methylases/genetics; DNA-Cytosine Methylases/isolation & purification; Escherichia coli/genetics; Escherichia coli Proteins; Genetic Vectors/genetics; Molecular Sequence Data; T-Phages/genetics |
| edit table |
Significance
You can help EcoliWiki by summarizing why this paper is useful
Useful Materials and Methods
You can help Ecoliwiki by describing the useful materials (strains, plasmids, antibodies, etc) described in this paper.
Annotations
<annotationlinks/>
EcoliWiki Links
Add links to pages that link here (e.g. gene, product, method pages)
References
See Help:References for how to manage references in EcoliWiki.
