PMID:1311302
| Citation |
Pucci, MJ, Discotto, LF and Dougherty, TJ (1992) Cloning and identification of the Escherichia coli murB DNA sequence, which encodes UDP-N-acetylenolpyruvoylglucosamine reductase. J. Bacteriol. 174:1690-3 |
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| Abstract |
The murB gene, which complemented the UDP-N-acetylenolpyruvoylglucosamine reductase (EC 1.1.1.158) mutation in Escherichia coli ST5, was cloned from an E. coli chromosomal library. murB was subcloned on a 2.8-kb PvuII fragment into pUC19 and sequenced. A 1,029-bp open reading frame encoded a 342-amino-acid polypeptide of 37,859 Da. A DNA sequence homology search revealed that murB had almost 100% homology with a previously reported unidentified open reading frame, ORFII, at 89.9 min. Physical and genetic mapping results were consistent with this map position, and minicell analyses of murB subclones showed a plasmid-encoded protein of approximately 37,000 Da, which closely matched the calculated size of the murB protein. |
| Links | |
| Keywords |
Carbohydrate Dehydrogenases/genetics; Chromosome Mapping; DNA Transposable Elements/genetics; Escherichia coli/enzymology; Escherichia coli/genetics; L Forms/genetics; Plasmids/genetics |
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