PMID:1309529
| Citation |
Løbner-Olesen, A and Marinus, MG (1992) Identification of the gene (aroK) encoding shikimic acid kinase I of Escherichia coli. J. Bacteriol. 174:525-9 |
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| Abstract |
DNA sequence analysis has revealed that an unidentified open reading frame (ufr1) is present immediately preceding the aroB gene of Escherichia coli. The predicted protein product of urf1 contains a consensus ATP-binding-site sequence and shows 34% amino acid homology to shikimate kinase II in a 97-amino-acid region. Inactivation of urf1 by insertion of an antibiotic resistance gene had a polar effect on aroB, indicating that these two genes constitute a transcriptional unit. The auxotrophic requirements of a strain mutant for both urf1 and aroL (encoding shikimate kinase II) are consistent with shikimate kinase deficiency. We propose that urf1 encodes shikimate kinase I and that it be designated aroK. |
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| Keywords |
Amino Acid Sequence; Base Sequence; Chromosomes, Bacterial; Cloning, Molecular; DNA Transposable Elements; Escherichia coli/enzymology; Escherichia coli/genetics; Escherichia coli/growth & development; Genes, Bacterial; Lac Operon; Molecular Sequence Data; Mutation; Open Reading Frames; Phosphotransferases/genetics; Phosphotransferases (Alcohol Group Acceptor); Transcription, Genetic |
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