PMID:12970756
| Citation |
Schreiter, ER, Sintchak, MD, Guo, Y, Chivers, PT, Sauer, RT and Drennan, CL (2003) Crystal structure of the nickel-responsive transcription factor NikR. Nat. Struct. Biol. 10:794-9 |
|---|---|
| Abstract |
NikR is a metal-responsive transcription factor that controls nickel uptake in Escherichia coli by regulating expression of a nickel-specific ATP-binding cassette (ABC) transporter. We have determined the first two structures of NikR: the full-length apo repressor at a resolution of 2.3 A and the nickel-bound C-terminal regulatory domain at a resolution of 1.4 A. NikR is the only known metal-responsive member of the ribbon-helix-helix family of transcription factors, and its structure has a quaternary arrangement consisting of two dimeric DNA-binding domains separated by a tetrameric regulatory domain that binds nickel. The position of the C-terminal regulatory domain enforces a large spacing between the contacts that each NikR DNA-binding domain can make with the nik operator. The regulatory domain of NikR contains four nickel-binding sites at the tetramer interface, each exhibiting a novel square-planar coordination by three histidines and one cysteine side chain. |
| Links |
PubMed Online version:10.1038/nsb985 |
| Keywords |
Amino Acid Sequence; Crystallography, X-Ray; Escherichia coli/chemistry; Escherichia coli/enzymology; Escherichia coli/metabolism; Escherichia coli Proteins; Molecular Sequence Data; Nickel/metabolism; Protein Structure, Tertiary; Repressor Proteins/chemistry; Repressor Proteins/metabolism |
| edit table |
Significance
You can help EcoliWiki by summarizing why this paper is useful
Useful Materials and Methods
You can help Ecoliwiki by describing the useful materials (strains, plasmids, antibodies, etc) described in this paper.
Annotations
<annotationlinks/>
EcoliWiki Links
Add links to pages that link here (e.g. gene, product, method pages)
References
See Help:References for how to manage references in EcoliWiki.